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350 Likewise, FGF21 analogs reduce urinary albumin excretion, mesangial expansion, and oxidative stress in db/db mice, linking their metabolic and antifibrotic actions to a promising DKD therapy

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A total of 20 g protein was separated using 10% or 12.5% sodium dodecyl sulphate polyacrylamide gel electrophoresis and blotted onto polyvinylidene fluoride membranes, which were then blocked with 5% non-fat milk diluted in Tris-buffered saline containing 0.05% Tween 20 for 1 h at room temperature

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Vohra et al., 2019)